Hijacked then lost in translation: the plight of the recombinant host cell in membrane protein structural biology projects

Hijacked then lost in translation: the plight of the recombinant host cell in membrane protein structural biology projects
Bill & von der Haar, 2015 - COSB

• "High quality protein" required for membrane protein crystallography
• Still difficult to obtain recombinant membrane protein in sufficient yield
• Use recombinant host cells - "cell factory" 
• Typically makes mutations to increase: 
• Protein yield
• protein stability
• Crystallization propensity

*Good review for MP experimental techniques