Kyrychenko et al. 2015 (Alexey Ladokhin) - Biophysical Journal
- pH Low insertion peptide (pHLIP) important tool for visualization & drug delivery
- Exists in three states: unfolded and soluble in water at neutral pH, unfolded and bound to surface of a PC membrane at neutral pH, and inserted across the membrane as an alpha helix at low pH
- Given anionic lipids, cholesterol or PE eliminates membrane binding at neutral pH
- pKa for insertion is increased with increasing anionic lipid content
- Suggests electrostatics at the interface modulate protonation important for insertion
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