Columbus, 2015 - COSB
- Four techniques used to facilitate MP expression:
- limited proteolysis - removes flexible regions
- Deglycosylation - removes heterogeneous post-translational modifications
- cysteine alkylation - prevents disulfide dimers and higher order oligomers
- lysine methylation
- Useful for determining more than 40 MP structures
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| (Figure 1 from Review) |
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